Research Topic: Protoplast isolation

PEG-Mediated Protoplast Transformation of Penicillium sclerotiorum (scaumcx01): Metabolomic Shifts and Root Colonization Dynamics

Scientists developed a new method to genetically modify a fungus called Penicillium sclerotiorum by using protoplasts, which are fungal cells with their protective outer walls removed. They added a glowing green protein (GFP) to track the fungus and discovered that this modification changed how the fungus uses fats and lipids. When they treated tomato seeds with enzymes before exposing them to the modified fungus, it enhanced the fungus’s ability to colonize plant roots, potentially helping plants grow better.

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Improved Protoplast Production Protocol for Fungal Transformations Mediated by CRISPR/Cas9 in Botrytis cinerea Non-Sporulating Isolates

Scientists have developed a better method to isolate protoplasts (fungal cells without cell walls) from non-sporulating varieties of gray mold fungus. By optimizing the incubation time, culture container, and enzyme used, they produced more viable protoplasts that can regenerate and be genetically modified. This advancement allows researchers to use CRISPR gene-editing technology to understand and potentially control gray mold, which causes significant crop losses worldwide.

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