molecular cloning – mycolab.ai

Novel acid trehalase belonging to glycoside hydrolase family 37 from Pleurotus sp.: cloning, expression and characterization

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Scientists discovered a new enzyme in Pleurotus mushrooms that breaks down trehalose, a special sugar that mushrooms use for growth and survival. This enzyme is unusual because it works in acidic conditions and belongs to a family of enzymes (GH37) that was previously thought only to contain neutral trehalases. The researchers cloned and produced this enzyme in laboratory yeast, then tested its properties to understand how it functions. This discovery helps explain how mushrooms manage their sugar metabolism during growth and decay.

Strategy of employing plug-and-play vectors and LC–MS screening to facilitate the discovery of natural products using Aspergillus oryzae

mycolabadmin

Researchers developed new tools to make it faster and easier to discover useful compounds from fungi. They created improved genetic vectors that allow scientists to insert multiple genes into Aspergillus oryzae more conveniently, and developed a quick screening method using mass spectrometry to identify successful transformants directly on culture plates. This approach saves about 10 days compared to traditional methods, significantly accelerating the discovery of new natural products with potential medical and agricultural applications.

Research Topic: molecular cloning

Novel acid trehalase belonging to glycoside hydrolase family 37 from Pleurotus sp.: cloning, expression and characterization

Strategy of employing plug-and-play vectors and LC–MS screening to facilitate the discovery of natural products using Aspergillus oryzae