long-read sequencing

Methodology for Extracting High-Molecular-Weight DNA from Field Collections of Macrofungi

mycolabadmin

Scientists developed a practical method to extract high-quality DNA from mushrooms collected in the wild, even in remote locations without refrigeration. The technique involves preserving fresh samples in alcohol and then carefully purifying the DNA through multiple steps. This method successfully produced DNA suitable for reading the complete genetic code of 23 different fungal species, particularly those that cannot be grown in laboratories.

Targeted long-read sequencing analysis and antifungal susceptibility profiles of Sporothrix schenckii isolates from Thailand

mycolabadmin

Researchers in Thailand studied a fungal infection called sporotrichosis caused by Sporothrix schenckii, which infects both cats and humans. Using advanced DNA sequencing technology from Oxford Nanopore, they identified and classified 26 fungal samples and tested how well common antifungal medications worked against them. They found that calmodulin gene sequencing was better than the standard ITS test for identifying the fungus species, and that about one-third of the samples showed reduced sensitivity to the main treatment drug, itraconazole.

Genome resource of Phlyctema vagabunda strain 19EL15, a pathogen of post-harvest bull’s eye rot of apple

mycolabadmin

Scientists sequenced the complete genetic blueprint of a fungus called Phlyctema vagabunda that causes serious damage to apples and pears after harvest, particularly creating brown spots called bull’s eye rot. The fungus is found across Europe and North America and costs farmers significant money in crop losses. This genetic information will help researchers better understand how the fungus works and develop better ways to prevent or manage the disease.

Targeted long-read sequencing analysis and antifungal susceptibility profiles of Sporothrix schenckii isolates from Thailand

mycolabadmin

This research examined a fungal infection called sporotrichosis found in Thailand, particularly in cats and humans. Scientists used advanced DNA sequencing technology to accurately identify which species of the fungus caused the infections and understand how they are related to each other. They also tested how well common antifungal medications work against these fungi, finding that some strains have become resistant to itraconazole, the main drug used to treat sporotrichosis.

Improved Extraction Methods to Isolate High Molecular Weight DNA From Magnaporthaceae and Other Grass Root Fungi for Long-Read Whole Genome Sequencing

mycolabadmin

Scientists have developed improved methods to extract high-quality DNA from pathogenic fungi, which is essential for sequencing and understanding how these fungi cause plant diseases. Two different extraction techniques were refined and tested on Magnaporthaceae fungi: one that works with small amounts of fungal material, and another that produces longer DNA strands when more material is available. The key to success is harvesting the fungi before they accumulate dark pigments that interfere with DNA extraction. These protocols will help researchers create better genome maps of disease-causing fungi, leading to improved strategies for crop protection.

Gapless near Telomer-to-Telomer Assembly of Neurospora intermedia, Aspergillus oryzae, and Trichoderma asperellum from Nanopore Simplex Reads

mycolabadmin

Researchers developed a new method to create complete, high-quality genetic maps of fungi using a single affordable sequencing technology from Oxford Nanopore. They created an automated computer program that processes the sequencing data without human intervention and successfully assembled complete genomes for three industrially important fungal species. This breakthrough could make it much easier and cheaper for scientists to study and use fungi for producing medicines, food ingredients, and other useful compounds.

Methodology for Extracting High-Molecular-Weight DNA from Field Collections of Macrofungi

mycolabadmin

Scientists developed a practical method to extract high-quality DNA from mushrooms found in nature, which is essential for understanding fungal genomes. The technique works without needing freezers or ice by preserving tissue in alcohol at room temperature. The method successfully extracted usable DNA from 33 different mushroom species, including rare species that cannot be grown in laboratories, enabling researchers to sequence and study their complete genomes.

Research Topic: long-read sequencing

Methodology for Extracting High-Molecular-Weight DNA from Field Collections of Macrofungi

Targeted long-read sequencing analysis and antifungal susceptibility profiles of Sporothrix schenckii isolates from Thailand

Genome resource of Phlyctema vagabunda strain 19EL15, a pathogen of post-harvest bull’s eye rot of apple

Targeted long-read sequencing analysis and antifungal susceptibility profiles of Sporothrix schenckii isolates from Thailand

Improved Extraction Methods to Isolate High Molecular Weight DNA From Magnaporthaceae and Other Grass Root Fungi for Long-Read Whole Genome Sequencing

Gapless near Telomer-to-Telomer Assembly of Neurospora intermedia, Aspergillus oryzae, and Trichoderma asperellum from Nanopore Simplex Reads

Methodology for Extracting High-Molecular-Weight DNA from Field Collections of Macrofungi