Research Topic: genome assembly

De novo Genome Assembly and Annotation of 12 Fungi Associated with Fruit Tree Decline Syndrome in ON, Canada

Apple and stone fruit trees in Ontario have been dying at alarming rates since 2016. Researchers sequenced and analyzed the genetic code of 12 different fungi suspected of causing this decline. This study provides the first complete genetic blueprints for several fungal species, helping farmers and scientists better understand what causes the disease and develop ways to prevent it.

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A high-quality genome assembly of angel-wing mushroom Pleurocybella porrigens that causes acute encephalopathy

Scientists created a detailed genetic blueprint of the angel-wing mushroom, which caused serious poisonings in Japan. They identified three toxic compounds in the mushroom and found a gene that likely produces one of the most dangerous toxins responsible for brain damage. This research helps explain how the mushroom makes its poison and could lead to better understanding of foodborne illness prevention.

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Genome assemblies for Pyricularia species and related genera isolated from diverse host plants

Scientists have sequenced the complete genetic blueprints of 35 fungal strains belonging to Pyricularia species and related genera, which cause devastating blast diseases in crops like rice and wheat. By analyzing genetic differences between strains that infect different grass species, researchers found that most Pyricularia oryzae strains show strong preferences for specific host plants. These new genome resources will help scientists understand how fungal pathogens evolve and adapt to new plant hosts, potentially improving disease management strategies.

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Haplotype-resolved genomes of Phlebopus portentosus reveal nuclear differentiation, TE-mediated variation, and saprotrophic potential

Scientists sequenced the complete genomes of two compatible strains of the king bolete mushroom (Phlebopus portentosus), an important edible and medicinal species. The study found that mobile DNA elements called transposons play a major role in creating genetic differences between the two fungal nuclei and in generating the diversity of compounds that give mushrooms their health benefits. The research shows this mushroom can both partner with trees and break down organic material on its own, making it uniquely adaptable.

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Complete mitochondrial genomic sequence of Auricularia delicata (Auriculariaceae), an edible Chinese mushroom

Scientists have sequenced the complete mitochondrial genome of Auricularia delicata, a popular edible jelly mushroom used in Chinese cuisine and traditional medicine. The genome is 189,696 base pairs long and contains 60 genes. This genetic information helps scientists understand how A. delicata is related to other mushroom species and provides a valuable resource for future research and cultivation of this important fungal species.

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Draft genome sequence of Neofusicoccum caryigenum associated with pecan leaf dieback

Scientists have sequenced the genetic blueprint of a fungus causing pecan leaf dieback disease, an emerging problem in southeastern U.S. pecan orchards. The fungus, called Neofusicoccum caryigenum, causes pecan trees to lose their leaves and produce fewer nuts. This first-ever genetic map of the pathogen will help researchers understand how it works and develop better ways to manage the disease.

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Haplotype-Phased Chromosome-Level Genome Assembly of Floccularia luteovirens Provides Insights into Its Taxonomy, Adaptive Evolution, and Biosynthetic Potential

Scientists have created the most detailed genetic map of the yellow mushroom (Floccularia luteovirens), a highly valued medicinal and edible fungus from the Tibetan Plateau. Using advanced sequencing technology, they mapped its 13 chromosomes and identified 15 pathways that the mushroom uses to make potentially useful healing compounds. The research also solved a long-standing mystery about the mushroom’s family tree, proving it is not actually related to Armillaria mushrooms as previously thought. This genetic blueprint opens new possibilities for developing medicines from this special fungus.

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Saprotrophic Arachnopeziza Species as New Resources to Study the Obligate Biotrophic Lifestyle of Powdery Mildew Fungi

Scientists have created a new way to study powdery mildew fungus, which causes widespread plant disease but cannot normally be studied in the laboratory. They identified a related fungus species (Arachnopeziza) that can grow in culture and can be genetically modified. By sequencing the DNA of these two Arachnopeziza species and developing methods to alter their genes, researchers have created a practical tool to understand how powdery mildew becomes dependent on its plant host, potentially leading to better disease control strategies.

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Comparison and Analysis of the Genomes of Three Strains of Botrytis cinerea Isolated from Pomegranate

Researchers compared three strains of gray mold fungus (Botrytis cinerea) that infect pomegranate fruits from different regions in Mexico. Using DNA sequencing and laboratory tests, they found that the MIC strain from Hidalgo was more aggressive at infecting fruit and breaking down plant tissues than the other two strains from the State of Mexico. These differences appear related to each strain’s genetic makeup and where they originated, which could help farmers develop better strategies to prevent gray mold disease on pomegranates.

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Whole-genome sequencing of global forest pathogen Diplodia sapinea causing pine shoot blight

Scientists have sequenced the complete genome of Diplodia sapinea, a fungus that causes serious disease in pine trees worldwide. This fungus normally lives harmlessly inside healthy pine trees but becomes dangerous during stressful conditions like droughts or storms. The new genome information will help scientists understand how this pathogen works and develop better ways to prevent and control the disease in forests.

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