Research Topic: gene silencing

Fusiform nanoparticle boosts efficient genetic transformation in Sclerotinia sclerotiorum

Scientists developed a new method using tiny fusiform nanoparticles to introduce genes into a destructive plant fungus called Sclerotinia sclerotiorum. This approach is simpler and faster than traditional genetic engineering methods because it doesn’t require complex cell preparation steps. The research shows that by silencing specific fungal genes, they could reduce the fungus’s ability to cause disease, which could help develop better strategies to protect crops like rapeseed and soybean.

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Argonaute1-Dependent LtmilR2 Negatively Regulated Infection of Lasiodiplodia theobromae by Targeting a Guanine Nucleotide Exchange Factor in RAS Signalling

Scientists discovered a small RNA molecule called LtmilR2 in a fungus that causes grape canker disease. This molecule naturally suppresses the fungus’s ability to infect grapes. By delivering this molecule or similar RNA duplexes to the fungus, researchers were able to inhibit its growth and infection, suggesting a new type of biological fungicide that could protect vineyards without chemical pesticides.

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Microbe-induced gene silencing of fungal gene confers efficient resistance against Fusarium graminearum in maize

Scientists developed a new method called microbe-induced gene silencing (MIGS) to protect maize crops from a destructive fungus called Fusarium graminearum, which causes stalk rot. They engineered a beneficial fungus (Trichoderma harzianum) to produce small RNA molecules that target and disable a critical gene in the pathogenic fungus, weakening its ability to infect plants. When maize seedlings were grown with this engineered beneficial fungus, they showed significantly better growth and reduced fungal infection compared to untreated plants. This approach offers an environmentally friendly alternative to chemical pesticides and does not require genetically modifying the crop itself.

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