Research Topic: gene expression

Targeted metabolomic and transcriptomic reveal the regulatory network of ultrasound on polyphenol biosynthesis in tender coconut flesh during storage

Researchers studied how ultrasound treatment affects the polyphenolic compounds (natural antioxidants) in coconut flesh during storage. By analyzing both the chemicals and genes involved, they found that ultrasound helps preserve important polyphenols like catechin and epicatechin by controlling the expression of genes that break them down. This discovery could help extend the shelf life of tender coconut products and maintain their nutritional value.

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Morphological and molecular development of Terfezia claveryi ectendomycorrhizae exhibits three well-defined stages

This study tracked how desert truffles form a beneficial partnership with plant roots over 10 weeks, identifying three distinct stages with different structures and genetic activity patterns. Researchers used microscopy and gene analysis to understand how the fungus gradually colonizes the root system, starting with growth in soil, then spreading between root cells, and finally penetrating inside cells. The findings reveal that specific fungal and plant genes are active at different stages, particularly those involved in breaking down plant cell walls. This research helps explain how desert truffles can be cultivated more effectively for food production.

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Transcriptomic changes in the PacC transcription factor deletion mutant of the plant pathogenic fungus Botrytis cinerea under acidic and neutral conditions

Gray mold is a common fungal disease affecting many plants, and it survives by adjusting to different pH levels in plant tissues. Scientists studied a specific protein called PacC that acts like a switch controlling which genes turn on or off based on acidity levels. By comparing normal fungi to mutants without this protein, researchers identified hundreds of genes that help the fungus adapt and cause disease, offering insights into how to potentially combat this agricultural problem.

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Transcriptome Analysis of Dimethyl Fumarate Inhibiting the Growth of Aspergillus carbonarius

Researchers found that dimethyl fumarate, a chemical preservative, can effectively stop the growth of a common fruit fungus called Aspergillus carbonarius that causes rot and produces a harmful toxin in grapes. By studying how the fungus responds to this treatment at the genetic level, scientists discovered that the chemical damages the fungus’s protective outer layer and interferes with its ability to develop and reproduce. This research could lead to better ways to preserve fruit and prevent toxin contamination in the food industry.

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Multi-omics analysis of Taiwanofungus gaoligongensis: effects of different cultivation methods on secondary metabolites

Scientists studied a medicinal mushroom called Taiwanofungus gaoligongensis to understand how different growing methods affect the production of beneficial compounds. They found that growing the mushroom in special bags with certain wood substrates produced far more of the valuable compounds (up to 12-fold more) compared to growing it on rice medium. By examining which genes were active in different growing conditions, they discovered how the mushroom’s cells control the production of these medicinal compounds, which could help farmers grow more potent medicinal mushrooms.

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Fruiting body-associated Pseudomonas contact triggers ROS-mediated perylenequinone biosynthesis in Shiraia mycelium culture

Scientists discovered that bacteria living inside medicinal mushroom fruiting bodies can trigger the production of powerful healing compounds called perylenequinones through direct physical contact. These compounds are being used to fight cancer and harmful bacteria through a therapy called photodynamic therapy. The study shows that when bacteria touch the mushroom’s cells, it causes the mushroom to produce more of these therapeutic compounds by creating controlled stress that activates specific genes.

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Inhibitive effect of Urginea epigea methanolic extract and silver/zinc oxide nanoparticles on Aspergillus and aflatoxin production

Researchers tested whether a plant called Urginea epigea could stop the growth of a dangerous fungus (Aspergillus flavus) that produces aflatoxins, which can harm humans and animals. The plant extract completely stopped fungus growth at high concentrations and significantly reduced the production of toxins by turning off the genes responsible for toxin production. This suggests that plant-based treatments could offer a natural alternative to synthetic chemical fungicides for protecting food from contamination.

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Transcriptome analysis of Ochratoxin A (OTA) producing Aspergillus westerdijkiae fc-1 under varying osmotic pressure

This research studied how salt concentration affects the production of ochratoxin A, a toxic substance produced by the fungus Aspergillus westerdijkiae that contaminates foods like coffee and grapes. Using advanced genetic analysis, scientists found that moderate salt levels (20 g/L) increase the fungus’s ability to produce this toxin by affecting specific genes. The findings help explain why OTA contamination is more common in salty foods like cured meats and suggest new ways to prevent this contamination and protect food safety.

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In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerio

Scientists tested a combination of beneficial bacteria (Lactobacillus strains) mixed with mushroom extract as a food supplement for farmed fish. After 4 months of feeding this supplement to zebrafish, the fish grew faster, converted food more efficiently, and showed improved reproductive capability with increased egg production. The mushroom extract helped the beneficial bacteria thrive and function better, suggesting this natural supplement could improve fish farming outcomes.

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Effects of Temperature, pH, and Relative Humidity on Growth of Penicillium crustosum OM1 Isolated from Pears and Its Penitrem A Production

Researchers found that a mold called Penicillium crustosum produces a dangerous neurotoxin called penitrem A that can contaminate pears and other foods. The study showed that this mold grows best at cool temperatures (25°C) but produces the most toxin at slightly cooler temperatures (22°C) with neutral acidity and high moisture levels. Understanding these conditions helps us develop better strategies to prevent this toxin from contaminating the fresh fruit we eat.

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