Research Topic: Real-time PCR

Epidermophyton floccosum, an etiological agent of tinea pedis and tinea unguium: about two cases

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Two elderly patients were diagnosed with fungal nail and foot infections caused by Epidermophyton floccosum, a fungus that has become less common in recent years. Both cases were confirmed using advanced molecular testing (real-time PCR) alongside traditional laboratory methods. The infections were successfully treated with terbinafine, an antifungal medication. This case report highlights the importance of using modern diagnostic techniques to accurately identify and treat fungal infections.

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Design of a melting curve analysis (MCA) based on multiplex real-time PCR for detection of Aspergillus terreus and Aspergillus fumigatus in cereals and oilseeds samples

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This study developed a fast molecular test using real-time PCR to detect harmful Aspergillus fungi in grains and seeds. Instead of waiting 3-5 days for traditional culture methods, this new test can identify the fungi in just hours by detecting specific DNA patterns. The test was tested on 140 samples of cereals and oilseeds and proved more accurate than traditional methods, making it useful for food safety in markets and processing plants.

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Dual-Emission FRET-PCR Outperforms SYBR Green and EvaGreen for Accurate Discrimination of Primary Canine Dermatophytes: Microsporum canis, Nannizzia gypsea, and Trichophyton mentagrophytes

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This research developed a new rapid test called FRET-PCR that can identify three common fungal infections in dogs within 2.5 hours, compared to 2-4 weeks for traditional culture methods. The test works by detecting specific genetic markers in fungal DNA and measuring temperature changes that differ for each fungal species. When compared to two other molecular tests (SYBR Green and EvaGreen), the FRET-PCR method was more accurate and sensitive, even able to detect a single copy of the target gene. This faster, more accurate identification will help veterinarians choose the right treatment more quickly and prevent spread of fungal infections.

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Design of a melting curve analysis (MCA) based on multiplex real-time PCR for detection of Aspergillus terreus and Aspergillus fumigatus in cereals and oilseeds samples

mycolabadmin

This study developed a fast molecular test to detect harmful fungi (Aspergillus species) in grains and seeds. Traditional fungal identification takes 3-5 days, but the new PCR-based method can produce results in hours. Testing 140 grain and seed samples, the new method successfully identified two dangerous Aspergillus species that produce toxic compounds harmful to human health. This advancement helps ensure food safety by enabling quicker detection of contamination in food production facilities.

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Epidermophyton floccosum, an etiological agent of tinea pedis and tinea unguium: about two cases

mycolabadmin

Two elderly patients with toenail and foot fungal infections caused by Epidermophyton floccosum were diagnosed using modern molecular testing methods. Traditional microscopic examination alone was difficult, but real-time PCR provided accurate identification. Both patients were treated with terbinafine, a common antifungal medication. The study highlights how modern DNA testing improves diagnosis compared to older methods.

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Dual-Emission FRET-PCR Outperforms SYBR Green and EvaGreen for Accurate Discrimination of Primary Canine Dermatophytes: Microsporum canis, Nannizzia gypsea, and Trichophyton mentagrophytes

mycolabadmin

Dogs often get fungal skin infections caused by three main types of fungi. Doctors have traditionally grown these fungi in culture, which takes 2-4 weeks. Scientists have now developed a faster genetic test called FRET-PCR that can identify which fungus is causing the infection in just 2.5 hours. This new test is more accurate and reliable than older genetic tests, helping veterinarians treat infections quickly and prevent them from spreading.

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Multicenter performance evaluation of the “quanty TOXO (RH region)” kit (Clonit) for molecular diagnosis of toxoplasmosis

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Researchers from seven French hospitals tested a commercial DNA test kit called “quanty TOXO” that detects Toxoplasma gondii, a parasite causing serious infections in pregnant women and people with weakened immune systems. The kit performed well overall, correctly identifying infection in nearly 95% of positive cases and showing no false positives. However, the test sometimes missed infections when parasite levels were very low, suggesting doctors should run tests twice to increase detection chances.

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