Research Topic: PCR-based detection

Clinical and Molecular Characterization of Feline Sporotrichosis in the Brazilian Amazon: PCR-Based Identification of Sporothrix brasiliensis

mycolabadmin

This research studied a fungal disease called sporotrichosis that affects cats in the Brazilian Amazon and can spread to humans. Most infected cats were young males that roamed freely outdoors and had skin lesions on their faces and paws. Researchers tested different laboratory methods to identify the fungus and found that a specific technique extracted DNA best. They confirmed that Sporothrix brasiliensis was the cause and tested how well common antifungal medications work against it, finding that some drugs work better than others.

Read More »

Identification of matB used as an endogenous reference gene for the qualitative and real-time quantitative polymerase chain reaction detection of Lentinus edodes

mycolabadmin

This study identifies the matB gene as a reliable genetic marker for detecting shiitake mushrooms (Lentinus edodes) in food products. Researchers developed a simple DNA test that can identify L. edodes in processed foods where it might be fraudulently substituted for more expensive wild mushrooms. The test is highly sensitive, detecting DNA at extremely low concentrations, and works on both raw mushrooms and processed products. This method provides food manufacturers and regulators with an effective tool to prevent mushroom fraud and ensure food authenticity.

Read More »

Diagnostic Approaches to Invasive Candidiasis: Challenges and New Perspectives

mycolabadmin

Invasive candidiasis is a dangerous fungal infection affecting critically ill hospital patients. Current testing methods like blood cultures are slow and unreliable, so doctors are developing faster tests using DNA detection and biomarkers. A combination of new diagnostic tools and prompt treatment with antifungal medications is essential to improve survival rates and reduce complications from this serious infection.

Read More »
Scroll to Top