therapeutic action: resistance assessment methodology

A rapid and efficient in vivo inoculation method for introducing tree stem canker pathogens onto leaves: suitable for large-scale assessment of resistance in poplar breeding progeny

Scientists developed a new, faster way to test poplar trees for resistance to stem canker diseases by inoculating fungal pathogens directly onto leaves instead of using whole stem segments. This method is much simpler, cheaper, and produces results in just 5 days compared to weeks for traditional methods. By testing leaves at different positions on trees and under different light conditions, researchers showed this leaf-based method accurately identifies resistant poplar clones for breeding programs.

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