This comprehensive review examines how scientists are fighting dangerous fungal infections that form protective biofilms resistant to current antifungal drugs. Researchers are using advanced protein analysis techniques (proteomics) and artificial intelligence-based computational tools to identify new targets for drug development against four critical fungal pathogens that cause life-threatening infections like meningitis and lung infections. By combining these technologies, scientists can better understand how these fungal biofilms form and develop more effective treatments.