This study shows that a beneficial fungus called Pochonia chlamydosporia can effectively control harmful root-knot nematodes that damage eggplant crops. When applied to soil, this fungus directly attacks nematode eggs and parasitizes the parasitic juveniles, while also boosting the plant’s natural defense systems. Not only does the fungus reduce nematode populations by over 55%, but it also helps eggplant plants grow better with increased height, weight, and chlorophyll content, providing a promising eco-friendly alternative to chemical pesticides.