This research studied a fungal disease called sporotrichosis that affects cats in the Brazilian Amazon and can spread to humans. Most infected cats were young males that roamed freely outdoors and had skin lesions on their faces and paws. Researchers tested different laboratory methods to identify the fungus and found that a specific technique extracted DNA best. They confirmed that Sporothrix brasiliensis was the cause and tested how well common antifungal medications work against it, finding that some drugs work better than others.