This research investigated natural alternatives to antibiotics in chicken feed, specifically testing mushroom extracts, different forms of copper, and lysozyme. The study aimed to find safer, more natural ways to protect chicken health without contributing to antibiotic resistance. Impacts on everyday life: – Helps develop safer food production methods by reducing antibiotic use in livestock – Contributes to addressing global antibiotic resistance concerns – Supports development of natural feed supplements for healthier animal products – Demonstrates potential for mushroom-based supplements in animal health – Could lead to more sustainable and environmentally friendly farming practices