Researchers successfully grew truffle fungi in the laboratory by isolating them from the roots of fir trees in Japanese forests. They identified eight different truffle species or lineages and studied how they grew on nutrient agar plates, finding that while they shared basic characteristics like white filamentous colonies, they had many differences in growth rates and hyphal structure. The study shows that collecting truffles from tree roots can be an effective way to obtain pure cultures of these fungi when fruiting bodies cannot be found.