This research developed a new molecular testing method to detect harmful fungi that cause green mold disease in commercially grown king oyster mushrooms. The test can identify multiple species of the fungus Trichoderma that damage mushroom crops, even at very low levels of contamination. Impacts on everyday life: – Helps mushroom farmers detect contamination earlier, leading to better crop protection – Could result in more stable mushroom supplies and pricing for consumers – Reduces crop losses and food waste in commercial mushroom production – Enables more efficient and targeted use of disease control measures – Supports sustainable cultivation of nutritious specialty mushrooms