This research investigated how different species of Trichoderma fungi affect the cultivation of medicinal mushroom Ganoderma lucidum. The study found that these fungi can significantly interfere with mushroom production by competing for resources and producing growth-inhibiting compounds. Impact on everyday life: • Affects the availability and cost of medicinal mushroom products used in traditional medicine • Influences sustainable farming practices for specialty mushroom growers • Demonstrates the complexity of managing agricultural systems • Provides insights for developing better mushroom cultivation methods • Has implications for natural product development and food security