This research investigated the types of bacteria present in the soil layer used to grow button mushrooms. The study discovered new bacterial species that had never before been found in mushroom growing materials. This finding is important for understanding how these bacteria might affect mushroom growth and production. Impacts on everyday life: • Better understanding of mushroom growing conditions could lead to improved mushroom production and availability • Insights into soil bacteria communities could help develop more effective growing methods • Knowledge gained could help farmers optimize mushroom yields • Could lead to more sustainable mushroom cultivation practices