This research investigated how a specific mushroom species (Pleurotus djamor) could be grown to produce beneficial compounds and tested their effectiveness against cancer. The study found that the mushroom extract was highly effective at inhibiting tumor growth in laboratory tests. This has important implications for everyday life: • Provides a potential new natural treatment option for certain types of cancer • Demonstrates how common mushrooms can be sources of powerful medicinal compounds • Shows promise for developing more sustainable and natural pharmaceutical products • Offers potential economic opportunities for mushroom cultivation industries • Highlights the importance of preserving biodiversity for medical research