This research discovered five new species of fungi living in freshwater environments in Thailand. The findings suggest there are many more undiscovered fungal species in Thai rivers and streams. Understanding fungal diversity is important because fungi play crucial roles in ecosystem health and may have potential applications in medicine and biotechnology. Impacts on everyday life: – Helps track biodiversity changes in aquatic ecosystems which affect water quality – Contributes to knowledge that could lead to discovery of new medicines or useful compounds – Improves understanding of decomposition processes in freshwater environments – Provides baseline data for monitoring environmental changes and ecosystem health – Advances scientific knowledge about Thailand’s natural heritage and biodiversity