This research investigated how a natural antifungal compound called gliotoxin, produced by beneficial soil fungi, behaves in different environmental conditions. This knowledge is important for developing effective biological crop protection strategies. The study found that the compound works best in slightly acidic soils that aren’t too wet, which helps farmers know when and where to apply these beneficial fungi for best results. Impacts on everyday life: • Helps farmers use natural fungicides more effectively to protect crops • Reduces the need for chemical pesticides in agriculture • Improves understanding of how natural compounds work in soil • Contributes to development of more sustainable farming practices • Provides insight into environmental factors affecting biological pest control