This research developed new molecular testing methods to detect and measure harmful bacteria that cause diseases in cultivated mushrooms. The tests are more accurate and sensitive than previous methods, allowing early detection of pathogens before they can cause significant crop damage. Impact on everyday life: – More reliable supply and quality of mushrooms for consumers – Reduced food waste from diseased mushroom crops – Lower production costs that could lead to more affordable mushrooms – Better food safety through early pathogen detection – More sustainable mushroom farming practices through targeted disease control