This research examined how a viral infection affects oyster mushroom growth and production. The study found that mushrooms infected with the PoV virus grew more slowly and produced fewer mushrooms due to reduced enzyme activity. This has important implications for commercial mushroom farming and enzyme production. Impacts on everyday life: • Could lead to better mushroom farming practices and higher yields • May help develop virus-free mushroom strains for improved production • Could improve industrial enzyme production from mushrooms • Helps understand how to protect food security in mushroom crops • May lead to more efficient and cost-effective mushroom cultivation