This research explored how to optimize the growth conditions of medicinal mushrooms to produce natural antibiotics. The study found that specific growing conditions could enhance the production of an antibacterial compound called isodrimenediol from the fungus Polyporus tricholoma, which shows promise in fighting certain bacterial infections. Impacts on everyday life: • Provides new ways to produce natural antibiotics that could help fight bacterial infections • Demonstrates potential for sustainable pharmaceutical production using mushrooms • Contributes to the development of alternative treatments for antibiotic-resistant bacteria • Shows how optimizing growth conditions can make natural medicine production more efficient • Highlights the untapped potential of mushrooms as sources of medical compounds