This research examined how genetic and epigenetic factors control development in two commercially important mushroom species. The study revealed that while DNA modifications remain largely stable during mushroom development, gene expression changes dramatically as mushrooms grow from thread-like structures into mature mushrooms. This has important implications for understanding and potentially improving mushroom cultivation. Impacts on everyday life: • Improved understanding of mushroom development could lead to better cultivation techniques • Knowledge gained could help develop more efficient production of edible mushrooms • Insights into fungal development could aid in breeding improved mushroom varieties • Better understanding of gene regulation in fungi has implications for biotechnology applications • Research advances our knowledge of how organisms develop and evolve