This research examined the genetic basis of how the medicinal mushroom Pleurotus tuber-regium forms its dense, nutrient-rich structures called sclerotia. By analyzing the mushroom’s genome and gene expression patterns, scientists identified key genes involved in this developmental process. This provides important insights into how medicinal mushrooms produce their beneficial compounds. Impacts on everyday life: – Helps improve cultivation methods for this nutritious and medicinal mushroom – Advances understanding of how to optimize production of natural medicines – Provides foundation for developing enhanced mushroom strains with better medicinal properties – Could lead to more efficient production of mushroom-based food ingredients and supplements – Contributes to preserving traditional medicinal knowledge through modern scientific validation