This research explored using agricultural waste materials to produce an important enzyme called phytase using mushrooms. The enzyme helps animals better absorb phosphorus from their food. The study found that the chestnut mushroom (Pholiota adiposa) could effectively produce this enzyme when grown on water hyacinth, a common aquatic weed. The researchers optimized the production conditions to get maximum enzyme yields. Impacts on everyday life: – Provides a way to convert agricultural waste into valuable products – Could lead to more sustainable and cost-effective animal feed supplements – Helps reduce environmental pollution from agricultural waste – Demonstrates potential for using invasive plants (water hyacinth) productively – Could lower costs of enzyme production for various industrial applications