Lentinula edodes – Page 10

Chemical Content and Cytotoxic Activity on Various Cancer Cell Lines of Chaga (Inonotus obliquus) Growing on Betula pendula and Betula pubescens

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This study examined chaga mushroom, a traditional medicine used against cancer, grown on two types of birch trees. Researchers tested chaga extracts on 31 different cancer cell types and found they were moderately effective at inhibiting cancer cell growth, particularly against liver and thyroid cancers. The chaga from silver birch (B. pendula) worked slightly better than that from downy birch (B. pubescens) due to higher levels of a compound called inotodiol.

On site discrimination between two closely related commercial strains of oyster mushroom using a loop-mediated isothermal amplification (LAMP) test

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Scientists developed a quick molecular test to identify two specific commercial oyster mushroom strains (SPOPPO and ALLERPO) in less than 30 minutes using a technique called LAMP. These sporeless mushroom strains were created to reduce respiratory health problems that workers develop from inhaling mushroom spores. The new test helps mushroom producers and breeders quickly verify strain identity in the field to prevent unauthorized copying of these valuable varieties and to catch products of suspect origin.

Providing a toolbox for genomic engineering of Trichoderma aggressivum

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Scientists have created a set of tools to genetically engineer Trichoderma aggressivum, a fungus that causes green mold disease in cultivated mushrooms but can also be used beneficially. The study provides step-by-step methods for transforming this fungus using both traditional plasmid methods and modern CRISPR gene-editing technology. These tools will help researchers understand how the fungus works and potentially harness its beneficial properties for agriculture.

GlSlt2 positively regulates GlMyb-mediated cellulose utilization in Ganoderma lucidum

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Scientists discovered how a medicinal mushroom called Ganoderma lucidum breaks down cellulose from plant waste. The study found that a protein called GlSlt2 activates another protein called GlMyb, which then turns on genes that produce cellulase enzymes. These enzymes break down cellulose into sugar that the fungus can use for growth. This discovery could help improve the conversion of agricultural waste into biofuels and other useful products.

Physicochemical Characterization and Antimicrobial Analysis of Vegetal Chitosan Extracted from Distinct Forest Fungi Species

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Researchers extracted and tested chitosan from five different mushroom species as an alternative to traditional crab-derived chitosan for allergic individuals. Using various scientific techniques, they found that chitosan from lion’s mane mushroom (H. erinaceus) had the best ability to dissolve in solution and kill bacteria, making it promising for food preservation. The study shows that mushroom-based chitosan can work as well as or better than shellfish-derived chitosan while avoiding allergen risks, offering a sustainable solution for food packaging and preservation.

Production of β-Glucans by Pleurotus ostreatus: Cultivation and Genetic Background

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Oyster mushrooms (Pleurotus ostreatus) are increasingly popular edible fungi that can grow on various waste materials like agricultural byproducts and food waste, making them both economical and environmentally friendly. These mushrooms produce valuable health-promoting compounds called β-glucans that have immune-boosting and antioxidant properties. Modern scientific techniques, including genetic analysis and artificial intelligence, are being used to optimize cultivation methods and increase production of these beneficial compounds. This sustainable approach to mushroom farming helps reduce waste while providing nutritious and medicinal food products.

Novel acid trehalase belonging to glycoside hydrolase family 37 from Pleurotus sp.: cloning, expression and characterization

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Scientists discovered a new enzyme in Pleurotus mushrooms that breaks down trehalose, a special sugar that mushrooms use for growth and survival. This enzyme is unusual because it works in acidic conditions and belongs to a family of enzymes (GH37) that was previously thought only to contain neutral trehalases. The researchers cloned and produced this enzyme in laboratory yeast, then tested its properties to understand how it functions. This discovery helps explain how mushrooms manage their sugar metabolism during growth and decay.

Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis

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Scientists studied mushroom cells to understand structures called lomasomes that form at the cell membrane surface. Using advanced microscopy techniques, they discovered these structures are made of tiny tubes and layers that can fold and swell into different shapes. These findings help explain how fungal cells that decompose wood organize their membranes and may be involved in how cells take in materials from their surroundings.

Characterization of Mycoviruses in Armillaria ostoyae and A. cepistipes in the Czech Republic

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Researchers in the Czech Republic discovered five new viruses infecting Armillaria mushroom fungi that cause root rot in trees. These viruses could potentially be used as biological control agents to reduce tree disease. The study found that some viruses can spread between different Armillaria species, suggesting they move naturally through forest ecosystems.

Mycelium Growth and Development of Psilocybe spp. Mother Cultures on Agar-Based Media

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This research examines how different growing media affect four types of psilocybin mushrooms. The study found that malt extract agar worked best for some mushroom types while potato-based media worked better for others. Commercial powdered potato media performed poorly, especially for one sensitive strain. These findings help optimize mushroom cultivation for therapeutic and commercial purposes.

Fungal Species: Lentinula edodes