This research explored using golden needle mushroom waste as a natural alternative to antibiotics in chicken feed for egg-laying hens. The study found that adding mushroom waste to chicken feed improved egg quality and strengthened the hens’ immune systems without negatively affecting egg production. Impacts on everyday life: – Provides a sustainable way to produce healthier organic eggs without antibiotics – Offers an environmentally friendly solution for recycling mushroom waste – Helps reduce antibiotic use in food production, addressing antibiotic resistance concerns – Could lead to more affordable organic egg options for consumers – Demonstrates how natural alternatives can maintain animal health and food quality