This research presents a simple and cost-effective method for preserving medicinal mushroom cultures in laboratories. The technique involves storing mushroom tissue on paper disks in sterile water, keeping them viable for extended periods without expensive equipment or complicated procedures. This matters to everyday life in several ways: • Makes it easier and cheaper for laboratories to maintain stocks of medicinal mushrooms for research • Helps preserve mushroom species that could be used to develop new medicines • Enables more consistent production of mushroom-based compounds for medical treatments • Reduces the cost and complexity of maintaining fungal cultures in research settings • Supports ongoing research into mushrooms’ potential health benefits