This research documents the first discovery of the medicinal mushroom Ganoderma tropicum in Thailand and determines the best laboratory conditions for growing its mycelia. This fungus is known for producing various bioactive compounds with potential medicinal properties. Impacts on everyday life: – Identifies optimal growing conditions for potential commercial cultivation of this medicinal mushroom – Expands knowledge of medicinal mushroom diversity in Thailand – Contributes to understanding fungal biodiversity in tropical regions – Provides foundation for future research into medicinal compounds from this species