This research investigated how mushrooms respond to olive oil production waste by producing an important enzyme called beta-glucan synthase. The study found that certain mushroom species, especially Lentinula edodes (shiitake mushroom), can effectively utilize this waste material to enhance enzyme production. This has implications for both waste management and biotechnology applications. Impacts on everyday life: • Provides a potential solution for managing olive oil production waste • Demonstrates new ways to make valuable products from industrial waste • Could lead to more sustainable mushroom cultivation methods • Shows potential for reducing environmental pollution from olive oil production • May contribute to more efficient production of beneficial fungal compounds