A simple protocol for producing axenic seeds of Sorghum bicolor
Researchers developed a simple method to sterilize sorghum seeds by treating them with ethanol and bleach, which removes contaminating microbes while keeping seeds alive and able to grow. This approach is inexpensive, requires no special equipment, and is useful for scientists studying how microbes interact with plants. The method successfully eliminated harmful microbes from 98% of seeds tested while maintaining a reasonable germination rate of 63%.