This research examined the DNA structure of mitochondria (cellular powerhouses) in an edible mushroom species. The study revealed complex DNA arrangements that can switch orientations and identified mechanisms for genetic variation. This has implications for understanding how fungi evolve and adapt. Impacts on everyday life: – Helps improve cultivation of edible and medicinal mushrooms – Advances our understanding of how organisms store and maintain their genetic information – Provides insights that could be applied to developing better fungal-based products and medicines – Contributes to knowledge about cellular energy production and inheritance – Demonstrates how modern DNA sequencing can reveal previously unknown biological complexity