This research examines how different mushroom species in the Bolbitiaceae family produce and develop their spores. The study revealed important differences in spore formation patterns that help scientists better classify these fungi and understand their evolutionary relationships. This has implications for fungal taxonomy and our understanding of mushroom diversity. Impacts on everyday life: • Helps mycologists better identify and classify mushroom species • Contributes to our understanding of fungal evolution and diversity • Supports the development of better mushroom cultivation techniques • Aids in the identification of potentially edible or toxic mushroom species