This research developed a quick and accurate DNA-based test to verify if food supplements actually contain genuine Maitake mushroom as claimed on their labels. The test can detect very small amounts of Maitake DNA and distinguish it from other mushroom species, helping prevent fraud in the supplement industry. Impacts on everyday life: • Helps consumers know they are getting genuine Maitake supplements they paid for • Protects against fraudulent or mislabeled mushroom products in the marketplace • Enables better quality control throughout the supplement production process • Makes testing of supplements faster and more reliable • Could lead to similar tests for other valuable mushroom supplements