This research found that adding corn oil to the liquid fermentation of medicinal mushroom Antrodia cinnamomea significantly improved the production of valuable medicinal compounds called triterpenoids. The study optimized fermentation conditions and revealed how corn oil enhances fungal growth and metabolism at the molecular level. Impacts on everyday life: • Provides a more efficient way to produce natural medicinal compounds • Could help make traditional Asian medicines more accessible and affordable • Demonstrates how common cooking oils can be used to improve biotechnology processes • Advances understanding of fungal growth and metabolism • Could lead to development of better pharmaceutical products