This research investigated how a compound called EK100, extracted from a rare Taiwanese mushroom, can help improve exercise performance and reduce fatigue. The study found that mice given EK100 had better muscle strength, could exercise longer, and showed less signs of fatigue compared to untreated mice. The compound works by helping the body store and use energy more efficiently while reducing exercise-related stress markers. Impacts on everyday life: • Could help athletes and fitness enthusiasts improve their exercise performance naturally • May help reduce exercise-related fatigue and muscle damage • Shows potential as a safe supplement for enhancing physical endurance • Could benefit people looking to improve their workout results • Demonstrates how natural compounds can be developed into effective performance supplements