This research examined nine wild mushroom species from Colombia to understand their nutritional value and potential health benefits. The study found these mushrooms to be excellent sources of protein and fiber while being low in fat, making them potentially valuable food sources. The mushrooms also showed promising abilities to fight harmful bacteria and fungi, while acting as antioxidants. Impact on everyday life: • Could provide new natural food sources high in protein and fiber • May lead to new natural antimicrobial treatments for infections • Could be developed into natural food preservatives • Offers potential new ingredients for health supplements and functional foods • Provides scientific support for traditional mushroom-based medicines