This research examined the beneficial compounds and antioxidant properties found in different combinations of medicinal mushroom mycelia grown in liquid culture. The study revealed these mushroom cultures are rich sources of health-promoting compounds and possess significant antioxidant capabilities. Impacts on everyday life: • Provides new ways to produce beneficial mushroom compounds through controlled cultivation • Demonstrates potential for developing new natural antioxidant supplements • Offers insights for creating more effective medicinal mushroom products • Shows promise for natural food preservation and functional food development • Supports the development of sustainable production methods for medicinal compounds