This research analyzed toxic compounds in various species of Lepiota mushrooms using advanced laboratory techniques. The study found that some Lepiota species contain dangerous toxins called amatoxins at levels even higher than the notorious death cap mushroom (Amanita phalloides). This work helps improve our understanding of which mushroom species are dangerous and aids in mushroom poisoning diagnosis and treatment. Impacts on everyday life: • Helps identify which wild mushrooms are potentially lethal if consumed • Improves medical diagnosis and treatment of mushroom poisoning • Advances food safety by better understanding toxic mushroom species • Assists mushroom foragers and mycologists in species identification • Contributes to development of better toxin detection methods