This study developed a new analytical method to detect and measure organophosphate flame retardants (OPFRs) in water samples. Researchers tested whether certain fungi, particularly white-rot fungi like Ganoderma lucidum and Trametes versicolor, could break down these toxic chemicals that are difficult to remove by conventional water treatment. The results show these fungi can effectively degrade some OPFRs, offering a promising biological treatment option for contaminated wastewater.