transcriptome analysis

Investigation of Efficient Pullulan Synthesis Utilizing Huangjiu Lees as a Substrate

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Scientists have discovered a way to use Huangjiu lees, the waste material left over from making Chinese rice wine, to produce pullulan, a valuable biopolymer used in food and medicine. By evolving a special fungal strain through repeated exposure to these lees, they created a strain that produces pullulan much more efficiently. Using optimized fermentation conditions and a two-stage process with different microorganisms, they achieved significantly higher pullulan yields, making this previously discarded byproduct into a valuable resource.

Transcriptome and Metabolome Reveal Accumulation of Key Metabolites with Medicinal Properties of Phylloporia pulla

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Scientists studied a medicinal fungus called Phylloporia pulla to understand which health-promoting compounds it produces and how it makes them. Using advanced genetic and chemical analysis tools, they discovered the fungus produces beneficial compounds like steroids and triterpenoids that have anti-inflammatory and anti-cancer properties, with production peaking around the middle of the fungus’s growth cycle. They identified six key genes that control the production of celastrol, a particularly valuable compound with potential to treat diseases like Alzheimer’s and cancer. This research helps explain why this fungus has been used traditionally in medicine and provides guidance for growing it to maximize production of these beneficial compounds.

Genome-wide identification of PSKR genes in wheat and differential expression under abiotic stress conditions

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Scientists identified 149 genes related to phytosulfokine receptors (PSKR) in wheat that help plants respond to environmental stresses like drought, salt, cold, and heat. These genes are spread across wheat’s chromosomes and contain regulatory elements that control their expression in response to various stresses and plant hormones. The study provides important genetic resources for developing wheat varieties that are more resistant to environmental stress, which is crucial for maintaining crop productivity in changing climate conditions.

Transcriptome Analysis Reveals Mechanisms of Stripe Rust Response in Wheat Cultivar Anmai1350

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Researchers studied how a wheat variety called Anmai1350 defends itself against a fungal disease called stripe rust caused by Puccinia striiformis. By analyzing gene activity at different time points after infection, they discovered that the wheat plant’s immune system activates multiple defense strategies, including producing toxic molecules called reactive oxygen species and defensive compounds called phytoalexins that prevent the fungus from spreading. This research helps scientists understand how to breed wheat varieties that can naturally resist this damaging disease and maintain crop yields.

Biomimetic Catechol-Incorporated Polyacrylonitrile Nanofiber Scaffolds for Tissue Engineering of Functional Salivary Glands

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Scientists developed a new synthetic material made from tiny fibers that can grow functional salivary glands in the laboratory. The special fibers, called PAN-C, attract and hold important proteins that naturally help glands develop. When cells are grown on these fibers, they form working gland structures that produce saliva-related proteins, offering hope for treating dry mouth and similar conditions.

Cystathionine Gamma-Lyase Regulate Psilocybin Biosynthesis in Gymnopilus dilepis Mushroom via Amino Acid Metabolism Pathways

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Researchers studying the ‘magic mushroom’ Gymnopilus dilepis discovered that a specific enzyme called CTH plays a crucial role in producing psilocybin, the compound with potential antidepressant effects. By blocking this enzyme, they reduced psilocybin production, but adding a related compound called L-cysteine restored it. This finding suggests that controlling amino acid metabolism could help increase psilocybin production for medical use.

Light-responsive transcription factor CmOzf integrates conidiation, fruiting body development, and secondary metabolism in Cordyceps militaris

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Scientists studied a medicinal fungus called Cordyceps militaris and discovered that a protein called CmOzf acts as a master controller of several important processes. When light shines on the fungus, it activates CmOzf, which helps the fungus produce spores for reproduction through a specific genetic pathway. Interestingly, when CmOzf is blocked, the fungus produces fewer spores but makes more pigments and beneficial compounds. This discovery could help improve the production of medicinal compounds from this fungus and its use as a natural pest control agent.

Act1 out of Action: Identifying Reliable Reference Genes in Trichoderma reesei for Gene Expression Analysis

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Scientists studying the fungus Trichoderma reesei, which is used industrially to produce enzymes, found that the reference genes commonly used to measure gene activity (act1 and sar1) are not as reliable as previously thought. By analyzing genetic data from multiple studies, they identified two better reference genes called bzp1 and tpc1 that maintain more consistent expression levels across different growth conditions. Using these better reference genes will help researchers more accurately measure how genes are expressed in this important industrial fungus.

Impact of energy metabolism pathways in promoting phytoremediation of cadmium contamination by Bacillus amyloliquefaciens Bam1

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Researchers developed genetically modified bacteria (Bacillus amyloliquefaciens) that produce more energy to better survive in cadmium-contaminated soil. These enhanced bacteria can then help tomato plants absorb and remove cadmium pollution from the soil more effectively. The best-performing modified strain increased cadmium accumulation in tomatoes by nearly 1.9 times compared to the original bacteria, offering a promising biological solution for cleaning contaminated agricultural soils.

Genome-wide identification and transcriptome analysis of the cytochrome P450 genes revealed its potential role in the growth of Flammulina filiformis

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Scientists identified 59 cytochrome P450 genes in the golden needle mushroom (Flammulina filiformis), an economically important edible fungus. These genes appear to control the mushroom’s growth and development, particularly the elongation of the stalk. By understanding how these genes work, researchers can potentially improve mushroom cultivation and develop new varieties with better growth characteristics. This research provides valuable insights into the genetics of mushroom growth and development.

Research Keyword: transcriptome analysis