Research Keyword: substrate optimization

Acid Phosphatase Produced by Trichoderma harzianum in Solid Fermentation Using Millet

Researchers used a fungus called Trichoderma harzianum grown on millet grain to produce phosphatase enzymes, which help convert unavailable phosphorus in soil into forms that plants can use. By carefully controlling the amount of millet, moisture, and fungal starter culture, they achieved significantly higher enzyme production than previous methods. This inexpensive, sustainable approach could improve soil fertility and plant growth in agriculture.

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A Comprehensive Review on Studying and Developing Guidelines to Standardize the Inspection of Properties and Production Methods for Mycelium-Bound Composites in Bio-Based Building Material Applications

This review examines mycelium-based composites, which are innovative building materials made from fungal networks grown on agricultural waste. These eco-friendly materials are biodegradable and use less energy to produce than traditional construction materials. The authors analyze current testing methods and propose standardized production guidelines to ensure consistent quality, helping make these sustainable materials more reliable for widespread use in buildings and construction.

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Optimizing laboratory cultivation of wood-inhabiting fungi with emphasis on applied conservation

Researchers studied how to grow seven rare and endangered wood-dwelling mushrooms in the laboratory, which is important for saving these species from extinction. They tested different growing conditions including adding sawdust to growth media, adjusting temperatures, adding vermiculite to grain-based substrates, and using different types of wood. The study found that simple and inexpensive modifications like using birch wood and adding vermiculite significantly improved mushroom growth, making conservation and commercial cultivation more practical and affordable.

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Mixing oak and eucalyptus sawdusts improves shiitake (Lentinula edodes) yield and nutritional value

This research explores growing shiitake mushrooms on different wood sawdust mixtures available in Lebanon. A combination of eucalyptus and oak sawdust outperformed oak alone, producing more mushrooms with better nutritional content including higher protein and fiber. This discovery helps local farmers improve mushroom production using readily available materials while creating healthier, more nutritious food.

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Biodegradation of ramie stalk by Flammulina velutipes: mushroom production and substrate utilization

This study shows that mushroom farmers can use ramie plant stalks, which are normally considered waste from textile production, as a growing substrate for golden needle mushrooms (Flammulina velutipes). By mixing ramie stalk with other common ingredients like wheat bran and cottonseed hulls in the right proportions, researchers achieved mushroom yields higher than using traditional substrates alone. This discovery helps reduce farming costs while solving an environmental waste disposal problem.

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Influence of Culture Conditions on Bioactive Compounds in Cordyceps militaris: A Comprehensive Review

Cordyceps militaris is a medicinal fungus used traditionally for treating fatigue, boosting immunity, and managing various health conditions. This comprehensive review explains how different growing methods, nutrients, light, temperature, and substrate materials affect the production of beneficial compounds in the fungus. The findings show that mixing grains with insect materials and using specific light conditions can significantly increase production of cordycepin, the most promising compound for cancer and immune health. The review identifies that more standardized growing methods and better understanding of the fungus’s metabolism are needed to make large-scale production practical and affordable.

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The Effects of the Substrate Length and Cultivation Time on the Physical and Mechanical Properties of Mycelium-Based Cushioning Materials from Salix psammophila and Peanut Straw

Researchers developed eco-friendly cushioning materials by growing mushroom mycelium (fungal networks) on agricultural waste like willow and peanut straw. These materials match the performance of plastic foam used in packaging but are completely biodegradable and made from renewable resources. By adjusting how long the mushrooms grow and the size of the substrate pieces, scientists can control the final product’s strength, flexibility, and water resistance.

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Influences of substrate and tissue type on erinacine production and biosynthetic gene expression in Hericium erinaceus

Researchers studied how different growing conditions affect erinacine production in lion’s mane mushroom mycelium. They found that the type of nutrients provided (substrate) significantly influences which erinacines are produced, even when the genes responsible for making these compounds show similar activity levels. Mycelium produced much more erinacines than fruit bodies, and specific nutrients could be used to encourage production of specific beneficial compounds.

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Development of Biphasic Culture System for an Entomopathogenic Fungus Beauveria bassiana PfBb Strain and Its Virulence on a Defoliating Moth Phauda flammans (Walker)

Researchers developed an efficient two-stage fermentation process to mass-produce Beauveria bassiana PfBb, a fungus that kills defoliating moths damaging Ficus trees. The first stage grows the fungus in liquid to produce active spores, which are then transferred to solid substrates to produce hardy, long-lasting conidia. The optimized process produces high quantities of effective fungal spores that can be stored at refrigerator temperature for over a year while maintaining their pest-killing ability.

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