Research Keyword: selection markers

Providing a toolbox for genomic engineering of Trichoderma aggressivum

Scientists have created a set of tools to genetically engineer Trichoderma aggressivum, a fungus that causes green mold disease in cultivated mushrooms but can also be used beneficially. The study provides step-by-step methods for transforming this fungus using both traditional plasmid methods and modern CRISPR gene-editing technology. These tools will help researchers understand how the fungus works and potentially harness its beneficial properties for agriculture.

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Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker

Scientists have successfully developed a genetic engineering method for Madurella mycetomatis, the fungus that causes mycetoma, a serious tropical disease. They used a technique to remove the fungal cell wall and insert genes into the cells, creating strains that produce green fluorescent protein (GFP). This breakthrough enables researchers to better understand how this fungus causes disease and to develop new treatments.

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