Research Keyword: seed viability

A simple protocol for producing axenic seeds of Sorghum bicolor

This study presents a simple and inexpensive method for sterilizing sorghum seeds to remove all living microbes while keeping them viable for planting. The process involves soaking seeds in ethanol and bleach for short periods, followed by rinsing. Of 95 seeds tested, only 2 showed contamination, and over half successfully germinated, making this method practical for seed research.

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