response surface methodology

Production, optimization and characterization of esterase isolated from a new endophytic Trichoderma afroharzianum strain AUMC 16,433 and its applications in dye decolorization

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Scientists discovered a new type of fungus that produces an enzyme capable of breaking down synthetic dyes used in the textile industry. Using statistical optimization techniques, they enhanced the enzyme’s production and purified it to study its properties. The enzyme successfully removed various industrial dyes from solutions, with the highest effectiveness on malachite green dye. This discovery offers a promising natural solution to reduce environmental pollution caused by textile dye wastewater.

Effects of adding food ingredients rich in dietary fiber and polyphenols on the microstructure, texture, starch digestibility and functional properties of Chinese steamed bun

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Researchers created an improved version of Chinese steamed bun by adding dragon fruit, kudzu root, and a medicinal mushroom called Hericium erinaceus. This new bun has much more fiber and antioxidants, and importantly, it causes much smaller increases in blood sugar after eating compared to traditional steamed buns. The study shows it can help promote beneficial gut bacteria and may be beneficial for people managing diabetes and metabolic health.

Extraction Optimization and Bioactivity of Polysaccharides from Ganoderma leucocontextum Spores

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Researchers developed an improved method to extract beneficial polysaccharides from Ganoderma leucocontextum (white Ganoderma), a rare mushroom from the Tibetan plateau. The extracted compound GLSP-A1 showed strong anti-inflammatory effects by reducing inflammatory markers and reduced signs of aging in test organisms by decreasing harmful reactive oxygen species and cellular aging pigments. This work suggests the mushroom polysaccharide could potentially be used in dietary supplements to help slow aging.

Ultrasound-assisted extraction of neuroprotective antioxidants from Ganoderma lucidum

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This research studied how to best extract healing compounds from the reishi mushroom (Ganoderma lucidum), an important traditional Chinese medicine. Using advanced extraction techniques and computer modeling, scientists identified optimal conditions that doubled the amount of beneficial antioxidants obtained compared to conventional methods. They then tested these extracts on nerve cells in the laboratory, demonstrating that the extracts effectively protected cells from oxidative damage and injury.

Optimization of the Decolorization of the Reactive Black 5 by a Laccase-like Active Cell-Free Supernatant from Coriolopsis gallica

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Textile factories produce large amounts of colored wastewater containing harmful dyes like Reactive Black 5. Researchers optimized an enzyme-based treatment using laccase from a fungus called Coriolopsis gallica to remove these dyes from water. By carefully balancing enzyme concentration, pH, temperature, and a chemical booster called HBT, they achieved 82% dye removal in just 2 hours, offering a cleaner and more environmentally friendly alternative to traditional chemical treatments.

Screening and Optimization of Solid-State Fermentation for Esteya vermicola, an Entomopathogenic Fungus Against the Major Forest Pest Pine Wood Nematode

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Scientists optimized how to grow large quantities of a beneficial fungus called Esteya vermicola in solid fermentation, which kills the pine wood nematode pest that destroys pine forests. By testing different combinations of ingredients (wheat bran, corn flour, soybean flour, glucose, yeast extract, and magnesium sulfate) and environmental conditions (temperature, humidity, inoculation volume, and time), researchers increased fungal spore production more than 4-fold. These results provide a practical method for producing biopesticide products to protect pine forests from this damaging pest.

Optimization and antifungal efficacy against brown rot fungi of combined Salvia rosmarinus and Cedrus atlantica essential oils encapsulated in Gum Arabic

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Researchers have developed a natural product that protects wood from fungal decay by combining oils from rosemary and cedar trees and encapsulating them in Gum Arabic using specialized techniques. This nanoencapsulation process creates tiny protective particles that are much more stable and effective than the oils alone. When tested against wood-damaging fungi, this product showed remarkable antifungal power comparable to commercial fungicides. This innovation offers a safe, environmentally-friendly alternative to synthetic wood preservatives for building and construction applications.

Valorization of Hericium erinaceus By-Products for β-Glucan Recovery via Pulsed Electric Field-Assisted Alkaline Extraction and Prebiotic Potential Analysis

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This research shows how scientists can extract beneficial compounds called β-glucans from leftover Lion’s Mane mushroom material using electrical pulses and alkaline treatment. These extracted compounds work like prebiotics, feeding and promoting the growth of beneficial bacteria in your gut, which then produce healthy fatty acids that support digestive health. The method is environmentally friendly and could help food companies make functional ingredients from mushroom waste.

From consortium design to bioaugmented filters: scalable yeast-based strategies for lead remediation in water systems

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Lead contamination in water is a serious health problem worldwide. This research developed a solution using natural yeast strains from a river that can remove lead from water. Scientists optimized three different yeast types to work together and incorporated them into filters, achieving up to 99.97% lead removal. This sustainable, low-cost approach could make clean water more accessible globally, especially in resource-limited areas.

Chitosan extraction from Amanita phalloides: yield, crystallinity, degree of deacetylation, azo dye removal and antibacterial properties

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Scientists successfully extracted chitosan, a useful natural polymer, from the death cap mushroom (Amanita phalloides). This chitosan showed excellent ability to remove blue dye from water and killed harmful bacteria effectively. The extraction method was efficient and eco-friendly, making it a promising solution for cleaning polluted water and developing antibacterial products.

Research Keyword: response surface methodology