real-time PCR – Page 2

Design of a melting curve analysis (MCA) based on multiplex real-time PCR for detection of Aspergillus terreus and Aspergillus fumigatus in cereals and oilseeds samples

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This study developed a fast molecular test using real-time PCR to detect harmful Aspergillus fungi in grains and seeds. Instead of waiting 3-5 days for traditional culture methods, this new test can identify the fungi in just hours by detecting specific DNA patterns. The test was tested on 140 samples of cereals and oilseeds and proved more accurate than traditional methods, making it useful for food safety in markets and processing plants.

Overexpression of efflux pump and biofilm associated genes in itraconazole resistant Candida albicans isolates causing onychomycosis

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Researchers studied why some fungal nail infections caused by Candida albicans stop responding to the antifungal drug itraconazole. They found that resistant fungal cells have higher activity of genes that pump the drug out of cells and produce slimy protective coatings called biofilms. These resistant fungi also formed less dense biofilms when the drug was present from the start. Understanding these resistance mechanisms could help develop new treatments by targeting the pump systems or breaking down the protective biofilm layers.

Evaluation of MucorGenius® Mucorales Real-Time PCR Assay with Formalin-Fixed Paraffin-Embedded Tissue Specimens

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Researchers tested a rapid fungal detection test called MucorGenius® on tissue samples from trauma patients with serious fungal infections. The test successfully identified dangerous mold-like fungi in 92% of infected samples with no false positives. This quick test could help doctors diagnose and treat dangerous fungal wound infections faster in trauma patients.

High-Resolution Melting assays development for discrimination of fungal pathogens causing Grapevine Trunk Diseases

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Grapevine Trunk Diseases cause major losses in vineyards worldwide but are difficult to diagnose early because symptoms may not appear for years. This study developed fast and affordable DNA-based tests called HRM assays that can identify ten different disease-causing fungi in grapevines. These tests work by analyzing DNA melting patterns and can even detect infections before symptoms appear, helping vineyard managers make better decisions about plant treatment and removal.

Dual-Emission FRET-PCR Outperforms SYBR Green and EvaGreen for Accurate Discrimination of Primary Canine Dermatophytes: Microsporum canis, Nannizzia gypsea, and Trichophyton mentagrophytes

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This research developed a new rapid test called FRET-PCR that can identify three common fungal infections in dogs within 2.5 hours, compared to 2-4 weeks for traditional culture methods. The test works by detecting specific genetic markers in fungal DNA and measuring temperature changes that differ for each fungal species. When compared to two other molecular tests (SYBR Green and EvaGreen), the FRET-PCR method was more accurate and sensitive, even able to detect a single copy of the target gene. This faster, more accurate identification will help veterinarians choose the right treatment more quickly and prevent spread of fungal infections.

Design of a melting curve analysis (MCA) based on multiplex real-time PCR for detection of Aspergillus terreus and Aspergillus fumigatus in cereals and oilseeds samples

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This study developed a fast molecular test to detect harmful fungi (Aspergillus species) in grains and seeds. Traditional fungal identification takes 3-5 days, but the new PCR-based method can produce results in hours. Testing 140 grain and seed samples, the new method successfully identified two dangerous Aspergillus species that produce toxic compounds harmful to human health. This advancement helps ensure food safety by enabling quicker detection of contamination in food production facilities.

Epidermophyton floccosum, an etiological agent of tinea pedis and tinea unguium: about two cases

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Two elderly patients with toenail and foot fungal infections caused by Epidermophyton floccosum were diagnosed using modern molecular testing methods. Traditional microscopic examination alone was difficult, but real-time PCR provided accurate identification. Both patients were treated with terbinafine, a common antifungal medication. The study highlights how modern DNA testing improves diagnosis compared to older methods.

Study of the antagonistic relationship between gene expression biofilm of Aspergillus niger and Staphylococcus aureus that cause otomycosis

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Researchers studied how bacteria (S. aureus) and fungi (A. niger) interact when both infect the ear canal, a condition called otomycosis. They found that bacteria significantly suppress the fungus’s ability to form protective biofilms by reducing the expression of genes needed for fungal growth. This antagonistic relationship suggests that mixed infections might actually be easier to treat than pure fungal infections, offering new insights for managing ear infections.

Dual-Emission FRET-PCR Outperforms SYBR Green and EvaGreen for Accurate Discrimination of Primary Canine Dermatophytes: Microsporum canis, Nannizzia gypsea, and Trichophyton mentagrophytes

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Dogs often get fungal skin infections caused by three main types of fungi. Doctors have traditionally grown these fungi in culture, which takes 2-4 weeks. Scientists have now developed a faster genetic test called FRET-PCR that can identify which fungus is causing the infection in just 2.5 hours. This new test is more accurate and reliable than older genetic tests, helping veterinarians treat infections quickly and prevent them from spreading.

Performance Evaluation of Five Real-Time PCR Assays for the Detection of Candida auris DNA

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Candida auris is a dangerous fungal infection that spreads in hospitals and resists common antifungal medications. Researchers compared five different rapid tests (PCR assays) to see which ones best detect this pathogen. The study found that different tests vary widely in their ability to detect the fungus, with some being much more sensitive than others. The findings highlight the need for standardized testing methods worldwide to ensure hospitals can reliably identify and control C. auris infections.

Research Keyword: real-time PCR