This study identifies the matB gene as a reliable genetic marker for detecting shiitake mushrooms (Lentinus edodes) in food products. Researchers developed a simple DNA test that can identify L. edodes in processed foods where it might be fraudulently substituted for more expensive wild mushrooms. The test is highly sensitive, detecting DNA at extremely low concentrations, and works on both raw mushrooms and processed products. This method provides food manufacturers and regulators with an effective tool to prevent mushroom fraud and ensure food authenticity.