Research Keyword: protoplast isolation

Effect of Suberoylanilide Hydroxamic Acid and Phytosulfokine-Alpha on Successful Plant Regeneration from Embryogenic Callus-Derived Protoplasts of Garlic (Allium sativum L.)

Scientists developed a new method to grow garlic plants from isolated plant cells (protoplasts), overcoming previous challenges in this difficult technique. They used special chemicals called SAHA and phytosulfokine to help cells divide and develop into complete plants. The new protocol successfully regenerated over 1,100 garlic plants with a 70% success rate for surviving outside the laboratory, offering new possibilities for garlic breeding and genetic improvement.

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Saprotrophic Arachnopeziza Species as New Resources to Study the Obligate Biotrophic Lifestyle of Powdery Mildew Fungi

Scientists studied two species of fungi called Arachnopeziza that are closely related to powdery mildew fungi but can grow independently on simple lab media. By analyzing their complete genomes and developing techniques to genetically modify these fungi, researchers created a new tool for understanding how powdery mildew fungi became dependent on plants. This breakthrough allows scientists to study these harmful plant pathogens more effectively without having to work directly with the difficult-to-cultivate powdery mildew fungi.

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Improved Protoplast Production Protocol for Fungal Transformations Mediated by CRISPR/Cas9 in Botrytis cinerea Non-Sporulating Isolates

Scientists have developed a better method to isolate protoplasts (fungal cells without cell walls) from non-sporulating varieties of gray mold fungus. By optimizing the incubation time, culture container, and enzyme used, they produced more viable protoplasts that can regenerate and be genetically modified. This advancement allows researchers to use CRISPR gene-editing technology to understand and potentially control gray mold, which causes significant crop losses worldwide.

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