protein expression – mycolab.ai

Enhancing the Substrate Adaptability of Laccase through Ancestral Sequence Reconstruction for Applications in Mycotoxin Detoxification

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Researchers used evolutionary analysis to redesign a fungal enzyme called laccase to better detoxify harmful mold toxins found in grains. The new engineered enzyme (LacANC278) can break down both aflatoxin and zearalenone toxins much more effectively than the original enzyme, and converts them into less harmful substances. This enzyme works without expensive helper chemicals and works well at room temperature, making it practical for treating contaminated corn and other grains.

Impact of Hericium erinaceus and Ganoderma lucidum metabolites on AhR activation in neuronal HT-22 cells

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This study examined how two medicinal mushrooms, lion’s mane and Reishi, affect nerve cells in the brain. The researchers found that metabolites from these mushrooms don’t harm brain cells and actually boost protective proteins that support cell health and survival. The mushrooms appear to work through a cellular receptor called AhR, which helps facilitate communication between the gut and the brain. These findings suggest that these mushrooms may help support brain function and protect against neurological problems.

The Molecular Mechanism of Polysaccharides from Polygonatum cyrtonema Hua in Improving Hyperuricemia by Regulating Key Targets of Uric Acid Metabolism in Mice

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This research demonstrates that a traditional Chinese medicinal plant called Polygonatum cyrtonema contains beneficial polysaccharides that can help lower high uric acid levels in the blood, which is a major cause of gout and kidney disease. The study found that these polysaccharides work by reducing the enzyme that produces uric acid and helping the kidneys excrete more of it, while also reducing inflammation. Importantly, this natural treatment protected kidney tissue better than the standard pharmaceutical drug allopurinol, suggesting it could be a safer long-term option for managing high uric acid levels.

Enhanced extracellular production of laccase in Coprinopsis cinerea by silencing chitinase gene

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Scientists improved the production of laccase, an enzyme with industrial uses in detoxification and food processing, by genetically engineering mushroom cells to have stronger cell walls. By reducing the activity of genes that break down chitin in the cell wall, they created mushroom strains that could better withstand the stirring forces during fermentation, resulting in over twice as much enzyme production. This breakthrough could lead to cheaper, more efficient production of this useful green catalyst on an industrial scale.

iTRAQ-Based Quantitative Proteomic Analysis Reveals Proteomic Changes in Mycelium of Pleurotus ostreatus in Response to Heat Stress and Subsequent Recovery

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This study examined how oyster mushrooms respond to high temperatures using advanced protein analysis techniques. Researchers found that when mushroom mycelium was exposed to 40°C heat, it damaged cell membranes and changed the levels of hundreds of proteins. However, when the temperature returned to normal, the mushrooms could repair the damage and recover. Key proteins including heat shock proteins and stress-response enzymes played important roles in protecting the mushroom cells and helping them survive heat stress.

Proteomic Analysis of Coprinopsis cinerea under Conditions of Horizontal and Perpendicular Gravity

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Researchers studied how mushrooms respond to gravity using Coprinopsis cinerea, a common lab fungus. They found that while the fungal threads (mycelium) don’t respond to gravity, the fruiting bodies do by growing away from the direction gravity pulls. Using advanced protein analysis techniques, they identified 51 proteins that change their levels depending on gravity direction, suggesting that gravity response is connected to how mushrooms develop their fruiting bodies.

Comparative Multi-Omics Analysis and Antitumor Activity of Phylloporia crataegi and Phylloporia fontanesiae

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Researchers compared two types of medicinal fungi (Phylloporia crataegi and Phylloporia fontanesiae) to understand why one is better at fighting cancer. They used advanced techniques to examine the fungi’s chemicals, genes, and proteins, discovering that P. crataegi contains special compounds like trans-cinnamic acid that help kill cancer cells. This study provides important information for developing new cancer treatments from these fungi.

Comparative Multi-Omics Analysis and Antitumor Activity of Phylloporia crataegi and Phylloporia fontanesiae

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Two species of medicinal fungi were studied to understand why one was better at fighting cancer cells. Researchers analyzed the chemicals, genes, and proteins in both fungi and found that Phylloporia crataegi had much higher levels of cancer-fighting compounds and activated special cellular defense pathways that harm cancer cells. This research shows that medicinal fungi could be promising sources for developing new cancer treatments.

Comparative Multi-Omics Analysis and Antitumor Activity of Phylloporia crataegi and Phylloporia fontanesiae

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Researchers compared two types of medicinal fungi (Phylloporia crataegi and P. fontanesiae) to understand why one is more effective at fighting cancer. Using advanced molecular analysis techniques, they found that P. crataegi contains higher levels of cancer-fighting compounds and activates more genes related to cancer cell death. These findings suggest that these fungi could be promising sources for developing new cancer treatments.

Research Keyword: protein expression